Trystan Casey
Transepithelial electrical resistance (TEER) is a widely accepted quantitative technique used to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers. TEER is a valuable method for assaying in vitro barrier tissue integrity and is becoming an important measurement for body-on-a-chip barrier tissue devices due to its usefulness and non-invasive nature. The measurement concept is relatively straightforward, with TEER measurements performed by applying an AC electrical signal across electrodes placed on both sides of a cellular monolayer and measuring voltage and current to calculate the electrical resistance of the barrier.
Transepithelial Electrical Resistance (TEER) Characteristics and Values
| Characteristics | Values |
|---|---|
| Definition | A widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers |
| Measurement | Performed by placing electrodes on both sides of a cell layer grown on a semipermeable membrane, applying an AC signal, and measuring both current and voltage across the cell layer |
| Cell Culture Models | Blood-brain barrier (BBB), gastrointestinal (GI) tract, and pulmonary models |
| Electrode Configuration | Silver and silver/silver chloride (Ag/AgCl) electrodes with a signal frequency of 12.5 Hz |
| Current | Low current signal of approximately 10 µA |
| Voltage | Measured using silver/silver chloride (Ag/AgCl) electrodes |
| Resistance Calculation | Ohm's Law is used to determine resistance from the current and voltage passing through the cell layer |
| Sensitivity | Very sensitive and reliable method to confirm the integrity and permeability of the monolayer |
| Variability | Manual methods are highly labor-intensive and subject to variability from electrode placement and changing environmental factors |
| Advantages | Quick, label-free, non-invasive, and can be performed in real-time |
| Applications | Evaluating drug or chemical transport or diffusion, drug screening assays, body-on-a-chip devices, and monitoring the quality of oviduct epithelial cells |
Explore related products
What You'll Learn
- Transepithelial electrical resistance (TEER) is a widely accepted quantitative technique
- TEER is a valuable method for assaying in vitro barrier tissue integrity
- TEER is a rapid, non-invasive method for quantifying barrier tissue integrity
- TEER is a well-established method of evaluating and monitoring epithelial tissue
- TEER is a functional parameter to monitor the quality of oviduct epithelial cells
Transepithelial electrical resistance (TEER) is a widely accepted quantitative technique
TEER is a rapid, non-invasive method for quantifying barrier tissue integrity by measuring the electrical resistance across the tissue. The measurement concept is relatively straightforward, with TEER measurements performed by applying an AC electrical signal across electrodes placed on both sides of a cellular monolayer and measuring voltage and current to calculate the electrical resistance of the barrier. The classical setup for measurement of TEER consists of a cellular monolayer cultured on a semipermeable filter insert, which defines a partition for apical and basolateral compartments. The electrical resistance of the cellular monolayer, measured in ohms, is a quantitative measure of the barrier integrity.
TEER is often used with epithelial and endothelial cells in a monolayer as a strong indicator of cell barrier integrity and permeability. These indicators are often used in vitro with evaluating the transport of drug chemicals or drug screening assays. TEER is also used to assess the barrier function of epithelial cells on porous supports. When assessing the electrical impedance, a continuous current passes through the cells on both transcellular and paracellular paths. The transcellular resistance is primarily made up of the apical and basolateral plasma membrane, while the paracellular resistance is a result of cell-substrate and cell-cell contacts.
TEER is a widely used and commercially available measurement system known as an Epithelial Voltohmmeter (EVOM). It uses a pair of electrodes known as a STX2/“chopstick” electrode pair. Each stick of the electrode pair contains a silver/silver chloride pellet for measuring voltage and a silver electrode for passing current. The measurement procedure includes measuring the blank resistance of the semipermeable membrane only and measuring the resistance across the cell layer on the semipermeable membrane.
Understanding Electrical Specs: 208-230-1-60 Voltage and Frequency
You may want to see also
Explore related products
TEER is a valuable method for assaying in vitro barrier tissue integrity
Transepithelial/transendothelial electrical resistance (TEER) is a valuable method for assaying in vitro barrier tissue integrity. TEER is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers. TEER is a well-established method of evaluating and monitoring epithelial tissue in a non-destructive assay.
TEER is a rapid, non-invasive method for quantifying barrier tissue integrity by measuring the electrical resistance across the tissue. The measurement concept is relatively straightforward, with TEER measurements performed by applying an AC electrical signal across electrodes placed on both sides of a cellular monolayer and measuring voltage and current to calculate the electrical resistance of the barrier. The classical setup for measurement of TEER consists of a cellular monolayer cultured on a semipermeable filter insert that defines a partition for apical and basolateral compartments. For electrical measurements, two electrodes are used, with one electrode placed in the upper compartment and the other in the lower compartment, separated by the cellular monolayer.
TEER is often used with epithelial and endothelial cells in a monolayer as a strong indicator of cell barrier integrity and permeability. These indicators are often used in vitro when evaluating the transport of drug chemicals or drug screening assays. TEER is a very sensitive and reliable method to confirm the integrity and permeability of the monolayer. TEER measurements can be performed in real-time without cell damage and are generally based on measuring ohmic resistance or measuring impedance across a wide spectrum of frequencies.
TEER is a useful tool for studying the function of a barrier tissue, especially an in vitro cultured barrier tissue. The cell layer must have high integrity with established tight junctions connecting adjacent cells to produce passive transport similar to that found in vivo.
Understanding KVA: Transformer Ratings Explained by R. Baker Electrical
You may want to see also
Explore related products
TEER is a rapid, non-invasive method for quantifying barrier tissue integrity
Transepithelial electrical resistance (TEER) is a valuable method for assaying in vitro barrier tissue integrity. TEER is a rapid, non-invasive method for quantifying barrier tissue integrity by measuring the electrical resistance across the tissue. TEER is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers.
TEER is a well-established method of evaluating and monitoring epithelial tissue in a non-destructive assay. The confluence of the monolayer can be quickly determined and tracked in real time as the TEER measurement will rise as the gaps in the monolayer close. TEER is often used with epithelial and endothelial cells in a monolayer as a strong indicator of cell barrier integrity and permeability.
The classical setup for measurement of TEER consists of a cellular monolayer cultured on a semipermeable filter insert that defines a partition for apical (or upper) and basolateral (or lower) compartments. For electrical measurements, two electrodes are used, with one electrode placed in the upper compartment and the other in the lower compartment, separated by the cellular monolayer. The ohmic resistance can be determined by applying a direct current (DC) voltage to the electrodes and measuring the resulting current. The ohmic resistance is calculated based on Ohm's law as the ratio of the voltage and current.
TEER is a very sensitive and reliable method to confirm the integrity and permeability of the monolayer. TEER is also a widely accepted electrical parameter to assess barrier integrity and suitability of in vitro cellular barriers for transport studies. TEER measurement has advantages over traditional permeability measurements as a quick, label-free, and non-invasive method.
Electric Dreams: Shoes On, Ready to Go!
You may want to see also
Explore related products
TEER is a well-established method of evaluating and monitoring epithelial tissue
Transepithelial electrical resistance (TEER) is a widely accepted and well-established method of evaluating and monitoring epithelial tissue. TEER is a valuable tool for assessing the integrity and permeability of epithelial barriers in the body. It involves measuring the electrical resistance across a cellular monolayer, typically composed of epithelial and endothelial cells.
The classical setup for TEER measurement involves using two electrodes, placed on either side of a cellular monolayer, to measure the electrical resistance. This technique is often referred to as the "chopstick" method. The electrodes are separated by the cellular monolayer, and the resistance is calculated based on Ohm's law, which determines the ratio of voltage to current. It is important to note that direct current (DC) can damage both the cells and the electrodes, so an alternating current (AC) is typically applied.
TEER is particularly useful for studying the function of barrier tissues, especially in vitro cultured barrier tissues. By measuring the electrical resistance across the tissue, TEER can quantify the integrity of the barrier and provide insights into the transport of drugs or chemicals through the epithelial and endothelial cell layers. This makes it a crucial tool in pharmacology and drug development.
TEER has been applied to various barrier models, including the blood-brain barrier, gastrointestinal (GI) tract, and pulmonary models. It is also used to monitor the quality of cultured epithelial cells, such as oviduct epithelial cells, and to study the effects of different culture conditions on their electrical resistance. Additionally, TEER can be used to assess the impact of infections or diseases on epithelial barrier integrity, as in the case of HIV's effect on the endometrium and cervix.
In summary, TEER is a well-established and widely accepted method for evaluating and monitoring epithelial tissue. It offers a rapid, non-invasive, and quantitative approach to studying barrier integrity and permeability, making it an indispensable tool in various research and pharmaceutical applications.
Understanding Electrical Overload: Its Meaning and Implications
You may want to see also
Explore related products
TEER is a functional parameter to monitor the quality of oviduct epithelial cells
Transepithelial electrical resistance (TEER) is a functional parameter used to monitor the quality of oviduct epithelial cells cultured on filter supports. TEER is a well-established method of evaluating and monitoring epithelial tissue in a non-destructive assay. The strength and integrity of these barriers can be assessed by measuring the electrical resistance across the cell layer in vitro.
TEER is used to assess the barrier function of epithelial cells on porous supports. When assessing the electrical impedance, a continuous current passes through the cells on both transcellular and paracellular paths. The transcellular resistance is primarily made up of the apical and basolateral plasma membrane, while the paracellular resistance is a result of cell-substrate and cell-cell contacts. The confluence of the monolayer can be quickly determined and tracked in real-time as the TEER measurement will rise as the gaps in the monolayer close.
TEER is often used with epithelial and endothelial cells in a monolayer as a strong indicator of cell barrier integrity and permeability. These indicators are often used in vitro when evaluating the transport of drug chemicals or drug screening assays. The expression of tight junction proteins is necessary for the formation of the epithelial barrier, integrity, and polarity.
TEER is a straightforward quality indicator that could be routinely used to monitor the differentiation status of oviduct epithelial cells in vitro. Fully differentiated cultures possessed a moderate TEER between 500 and 1100 Ω*cm(2). Only 5% of cultures that exhibited TEER values in this defined range had poor quality.
Understanding Electric Arcs: Definition, Meaning, and Applications
You may want to see also
Frequently asked questions
Transepithelial electrical resistance (TEER) is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers.
TEER is traditionally measured by placing two "chopstick" style electrodes on either side of a transwell insert with a confluent cell layer.
TEER is used to evaluate and monitor epithelial tissue in a non-destructive assay. It is a strong indicator of cell barrier integrity and permeability.
Transepithelial resistance (RT or TER) is the resistance measurement taken from a cell layer. TEER is the method used to obtain this measurement.
Examples of TEER being used to measure the integrity of a barrier include the blood-brain barrier (BBB), gastrointestinal (GI) tract, and pulmonary models.
![Resistance [Blu-ray]](https://m.media-amazon.com/images/I/71BvypKgp3L._AC_UL320_.jpg)
![Resistance [DVD]](https://m.media-amazon.com/images/I/91yILWSanCL._AC_UL320_.jpg)
